MF2120019
Escherichia coli met repressor (MetJ)
1cmb
X-ray
1.80
homodimer
Escherichia coli
2677753
Rafferty JB, Somers WS, Saint-Girons I, Phillips SE
Three-dimensional crystal structures of Escherichia coli met repressor with and without corepressor.
Nature
1989
6244
341
705-10
The three-dimensional crystal structure of met repressor, in the presence or absence of bound corepressor (S-adenosylmethionine), shows a dimer of intertwined monomers, which do not have the helix-turn-helix motif characteristic of other bacterial repressor and activator structures. We propose that the interaction of met repressor with DNA occurs through either a pair of symmetry-related alpha-helices or a pair of beta-strands, and suggest a model for binding of several dimers to met operator regions.
GO:0003700
transcription factor activity, sequence-specific DNA binding
GO:0003677
DNA binding
GO:0009086
methionine biosynthetic process
GO:0006351
transcription, DNA-templated
GO:0045892
negative regulation of transcription, DNA-templated
GO:0005829
cytosol
No modifications of the original PDB file. Chain identifiers are identical with the PDB's identifiers.
2
1
Other
Other
A
Met repressor
Escherichia coli
AEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRRQVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPETWEY
104
P0A8U6
2
105
99%
UniRef90_A7ZUF7
2
105
secondary structure
beta
14
15
secondary structure
beta
18
19
secondary structure
beta
22
30
secondary structure
helix
31
47
secondary structure
beta
49
49
secondary structure
helix
54
67
secondary structure
helix
74
77
secondary structure
beta
78
78
secondary structure
helix
87
95
pfam
PF01340.17
MetJ
3
99
B
Met repressor
Escherichia coli
AEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRRQVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPETWEY
104
P0A8U6
2
105
99%
UniRef90_A7ZUF7
2
105
secondary structure
beta
22
30
secondary structure
helix
31
46
secondary structure
helix
54
67
secondary structure
beta
80
80
secondary structure
beta
83
83
secondary structure
helix
87
95
pfam
PF01340.17
MetJ
3
99
MetJ was shown by differential scanning calorimetry to follow a two-state folding and dimerization (PMID:1390748).
1cma
1cmc
1mj2
1mjk
1mjl
1mjm
1mjo
1mjp
1mjq