##################################################### # Mutual Folding Induced by Binding (MFIB) database # # Version: 26-06-2017 # # Source: http://mfib.enzim.ttk.mta.hu/ # ##################################################### Format definition for the downloadable version of MFIB (MFIB_complete.txt). Entries are separated by empty lines. Each entry consists of several lines, each of which starts with a [tag], followed by an equal sign (=), followed by the actual value. While most tags are present for every entry, some are optional. The definition of various data and their corresponding tags are listed below. [Entry] - Start of the entry. [Accession] - MFIB database accession. Consists of the characters 'MF' followed by 7 digits. [Name] - A short description of the protein complex presented in the entry. [Source organism] - The list of organisms from which the constituent protein chains originate. [Assembly] - The oligomeric state of the complex. Also describes whether the complex is hetero- or homo-oligomeric. [Total number of proteins] - The number of polypeptides involved in the described interaction. [Number of unique proteins] - The number of different polypeptides involved in the described interaction. [Class] - The MFIB class of the described complex (can be one of the following: 'Bulb-type lectin domain', 'Coils and zippers', 'Histone-like interactions', 'Homooligomeric enzymes', 'L27 domains', 'NGF-like proteins', 'Transthyretin-like folds', and 'Other'). [Subclass] - Further classification of the complex inside its respective class. For possible subclasses inside each class see the source website. [PDB ID] - ID of the PDB structure describing the complex. [PDB chain IDs] - The list of IDs for chains in the PDB structure that take part in the described interaction. [PDB note] - A description of the transformations made on the referred PDB structure to properly display the biologically relevant, core interaction. These transformations can be the omission of protein chains (to reduce possible duplicity present in the PDB structure), the generation of protein chains (based on the biomatrices described in the PDB file), or truncations of protein chains (to only include regions of proteins that mediate the highlighted interaction). [PDB experimental technique] - Experimental technique used to determine the structure described in the PDB. Can be 'X-ray' or NMR. [PDB resolution] - The resolution of the corresponding PDB structure in Angstom units (optional - only present if the experimental technique is X-ray). [Evidence global] - Description of and reference(s) to the exerimental work(s) that demonstrate that all of the constituent protein chains involved in the interaction loose their tertiary structure upon dissociation (i.e. the protein complex is formed from unstructured monomers that only adopt their 3D structure upon interaction). This field is optional but can only be missing if all interacting chains have separate evidence listed below. [Evidence chain X] - Description of and reference(s) to the exerimental work(s) that demonstrate that the region of chain X that is involved in the interaction is intrinsically disordered (i.e. it only adopts its structure upon interacting with its protein partners). This field is optional but can only be missing for any one chain in the interaction if [Evidence global] is present. [Chain X name] - The name of the protein with chain ID X (as defined in UniProt). [Chain X source organism] - The organism from which the protein with chain ID X originates. [Chain X UniProt accession] - UniProt accession of the protein with chain ID X. [Chain X UniProt boundaries] - The region of the UniProt sequence that is involved in the interation. [Chain X UniProt coverage] - The fraction of the UniProt sequence that is involved in the interation. [Chain X UniRef90 accession] - The UniRef90 cluster to which the protein with chain ID X belongs. [Chain X UniRef90 boundaries] - The region of the representative UniRef90 sequence that corresponds to the protein region with chain ID X involved in the interation. [Related structures] - PDB IDs of structures that contain interactions that are highly similar to the one described in the entry (for a detailed description of what qualifies as 'highly similar' see the MFIB paper or the Help section at the MIFB website. This field is optional and is omitted if there are no such related structures.