Database accession: MF2202001
Name: GTPase binding domain of neural Wiskott-Aldrich syndrome protein in complex with E. coli EspF(U)
PDB ID: 2lnh
Experimental method: NMR
Source organism: Homo sapiens / Escherichia coli O157:H7
Primer publication of the structure:
Aitio O, Hellman M, Skehan B, Kesti T, Leong JM, Saksela K, Permi P
Enterohaemorrhagic Escherichia Coli Exploits a Tryptophan Switch to Hijack Host F-Actin Assembly.
(2012) Structure :
Intrinsically disordered protein (IDP)-mediated interactions are often characterized by low affinity but high specificity. These traits are essential in signaling and regulation that require reversibility. Enterohaemorrhagic Escherichia coli (EHEC) exploit this situation by commandeering host cytoskeletal signaling to stimulate actin assembly beneath bound bacteria, generating "pedestals" that promote intestinal colonization. EHEC translocates two proteins, EspF(U) and Tir, which form a complex with the host protein IRTKS. The interaction of this complex with N-WASP triggers localized actin polymerization. We show that EspF(U) is an IDP that contains a transiently α-helical N-terminus and dynamic C-terminus. Our structure shows that single EspF(U) repeat forms a high-affinity trimolecular complex with N-WASP and IRTKS. We demonstrate that bacterial and cellular ligands interact with IRTKS SH3 in a similar fashion, but the bacterial protein has evolved to outcompete cellular targets by utilizing a tryptophan switch that offers superior binding affinity enabling EHEC-induced pedestal formation.
Molecular function: not assigned
Biological process: not assigned
Cellular component: not assigned
Entry contents: 2 distinct polypeptide molecules
Chains: A, C
Notes: Chain B was removed as it is ordered and does not directly contribute to the interaction between chains A and C. Chain C was truncated to exclude the region in contact with chain B.
Number of unique protein segments: 2
Name: Neural Wiskott-Aldrich syndrome protein
Source organism: Homo sapiens
Length: 65 residues
Sequence:Sequence according to PDB SEQRESGSNFQHIGHVGWDPNTGFDLNNLDPELKNLFDMCGISEAQLKDRETSKVIYDFIEKTGGVEAVKN
UniProtKB AC: O00401 (positions: 206-270)Coverage: 12.9%
UniRef90 AC: UniRef90_O00401 (positions: 207-270)
Name: Secreted effector protein EspF(U)
Source organism: Escherichia coli O157:H7
Length: 48 residues
Sequence:Sequence according to PDB SEQRESGLPDVAQRLMQHLAEHGIQPARNMAEHIPPAPNWPAPTPPVQNEQSRP
UniProtKB AC: P0DJ89 (positions: 220-267)Coverage: 14.2%
UniRef90 AC: UniRef90_P0DJ88 (positions: 221-267)
The 221-314 region described in IDEAL entry IID90008 covers 97.9% of the sequence present in the structure. The interacting region has also been shown to be disordered in the structure's source publication (PMID: 22921828).
No related structure was found in the Protein Data Bank.